Sutter 2P Rig Documentation
This documentation covers the setup, configuration, and operation of the Sutter 2P microscopy system for in vivo 2-photon calcium imaging (2PCI).
Documentation Sections
Reference
- About - Brief overview
- Hardware - Understand system components and wiring
- Software - Software installation and setup
- Drivers - Required drivers and installation
- Pupillometry - Details for capturing pupillometry
Alignment and calibration
- 2P Laser Alignment - Laser alignment procedures with safety protocols
- Speaker Calibration - Procedures for calibrating the free-field speaker
- Laser Power Calibration - Laser power measurement
Setup and installation
- Computer Setup - Install drivers and software to configure rig after reinstalling Windows
- Configuration - Ephus (widefield + sound stimuli) and ScanImage (2P) settings and detailed parameter explanations
Daily Operation
- Operation - Reference for acquisition loops etc.
Data analysis and operation code
- Code - Custom scripts and analysis tools
Support
Primary Contact: Rick Ayer (Sutter Instruments) Email: rick@sutter.com