Hardware

• Sutter 2P rig for in vivo 2PCI (2 Photon Calcium Imaging)

Computer

• Dell Precision Tower 5810
  • Intel Xeon CPU E5-1630 @ 3.70 GHz
  • 32.0 GB RAM
  • NVIDIA NVS 310 Graphics

Microscope

• Sutter MOM (Moveable Objective Microscope)
• Objective moves rather than the stage (stage moves on Scientifica rig)
• dichroic splitter (Di02-R561, Semrock)
• green emission filter (FF03-525/50, Semrock)
• objective: 40 0.8 NA objective (Olympus)

DAQs

1. NI USB-6229 (ID: Dev2)
   • Ephus acquire / sound stimulation
2. NI PCI-6110 (BNC-2090A breakout) (ID: Dev1)
   • ScanImage 2P:
     • Galvo Mirrors (Channels: AO0,AO1)​
     • PMT (Channels: AI8, AI9)
     • Shutter (Channels: USER 1)
     • Trigger output to trigger Ephus sound delivery (Channels: USER 2)
       • Path: PFI13 (Digital I/O PFI terminal block) ⇒ USER 2 ⇒ BNC cable ⇒ NI USB 6229 ⇒ PFI0/P1.0​ ⇒ PFI10 (terminal block)

PMT

• Controller: Sutter PS-2LV
• Hamamatsu H10770PA-40

Shutter

• Controller: ThorLabs SC10 Shutter Controller

Galvo / Scan controller

• Sutter MDR MOM Scan Drive Controller
• Galvo / Galvo mirrors for laser scanning

Laser

• MaiTai HP, Newport

Speaker and Speaker controller

• freefield speaker (ES1, Tucker Davis)
• ED1 speaker driver (Tucker Davis)

Micromanipulator

• Sutter ROE-200

Power Intensity Controller

• ThorLabs Kinesis Motor Controller KDC101
• Rotates PRM1Z8 to adjust laser intensity entering Sutter enclosure

Temperature control

• FHC DC Temperature controller with heat pad and rectal thermister